PERTANIKA JOURNAL OF SCIENCE AND TECHNOLOGY

This study focuses on the Pteroptyx tener species in the Sepetang River, Malaysia, aiming to evaluate the firefly’s abundance and explore its correlation with various biotic and abiotic parameters. The study was conducted over six months, from November 2021 to April 2022, utilizing Geographic Information System (GIS) software to apply hotspot mapping and Inverse Distance Weighting (IDW) analysis to elucidate the spatial distribution of firefly populations. A total of 111,615 individuals were recorded, with a particular focus on this firefly species’ presence on their display trees. Hotspot analysis showed that Station 6, located at the mouth of a river with dense mangroves, hosted 55,723 fireflies (50.01%). In contrast, Stations 9 and 10, near ponds and shrimp settlements, recorded 517–723 fireflies (0.65% and 0.46%). Pearson’s correlation coefficient (r) unveiled a statistically significant positive correlation (r = 0.88, p < 0.05) between wind speed and the abundance of firefly populations within the Sepetang River. However, no statistically significant correlation (p > 0.05) was found between firefly abundance and various other abiotic parameters, including relative humidity (RH), air temperature, tide level, pH, electrical conductivity (EC), salinity, total dissolved solids (TDS), and water clarity. Thus, the results revealed the preference for fireflies due to the availability of vegetation, wind speed and minimal disturbance in this area. In conclusion, this study’s information significantly adds to our understanding of these interesting insects and their complicated relationships in nature. It underscores the importance of preserving their habitats and ecosystems.

• Abdullah, N. A., Asri, L. N., Husin, S. M., Shukor, A. M., Darbis, N. D. A., Ismail, K., Agustar, H. K., Sulaiman, N., & Hazmi, I. R. (2021). Influence of physico chemical characteristics of rembau river, peninsular Malaysia, on congregating Pteroptyx fireflies. Environmental Monitoring and Assessment, 193(10), Article 634. https://doi.org/10.1007/s10661-021-09426-y

• Abdullah, N. A., Radzi, S., Asri, L. N., Idris, N. S., Husin, S., Sulaiman, A., Khamis, S., Sulaiman, N., & Hazmi, I. R. (2019). Insect community in riparian zones of Sungai Sepetang, Sungai Rembau and Sungai Chukai of Peninsular Malaysia. Biodiversity Data Journal, 7, Article e35679. https://doi.org/10.3897/BDJ.7.e35679

• Abdullah, N. A., Asri, L. N., Radzi, S. N. F., Musbah, M., Hazmi, I. R., & Sulaiman, N. (2020). Abiotic factors influencing diversity and abundance of congregating fireflies (Coleoptera: Lampyridae) in Miri, Sarawak, Malaysia. Oriental Insects, 55(2), 149-164. https://doi.org/10.1080/003053 16.2020.1757529

• Angelin, J. A., Jehamalar, E. E., Das, S. S. M., & Kumar, S. P. (2010). Effect of salinity on the distribution of aquatic insects of Manakudy estuary, Kanyakumari district. Journal of Basic and Applied Biology, 4, 91-97.

• Asri, L. N., Abdullah, N. A., Sulaiman, A., Asri, M. H. M., Norela, S., Satiman, E. M. F. E. N., Husin, S. M., Shukor, A. M., & Darbis, N. D. A. (2021). Abundance and species composition of synchronous flashing firefly at Sungai Rembau, Negeri Sembilan, Malaysia. International Journal of Tropical Insect Science, 41, 1095-1106. https://doi.org/10.1007/s42690-020-00295-5

• Asyraf, M. K. M., Nor’Aini, Y., & Suraiyati, R. (2013, October 29-31). Rivers, lakes, and swamps: Sustainable approach towards ecotourism. In Proceedings of the 3rd Regional Conference on Tourism Research (pp. 29-31). Langkawi, Malaysia.

• Atkins, V., Bell, D., Bowker, A., Charig, M., Crew, J., Dale, M., Hickmott, B., Payne,B., Pendleton, D., Pendleton, T., Robinson, M., Wollen, K., Woodell, B., & Tyler, J. (2017). The status of the glow-worm Lampyris noctiluca L. Coleoptera: Lampyridae) in England. Lampyrid, 4, 20-35.

• Axmacher, J. C., Brehm, G., Hemp, A., Tünte, H., Lyaruu, H. V., Müller‐Hohenstein, K., & Fiedler, K. (2009). Determinants of diversity in afrotropical herbivorous insects (Lepidoptera: Geometridae): Plant diversity, vegetation structure or abiotic factors? Journal of Biogeography, 36(2), 337-349. https://doi.org/10.1111/j.1365-2699.2008.01997.x

• Balaman, Ş. Y. (2019). Modeling and optimization approaches in design and management of biomass-based production chains. In Decision-making for Biomass-Based Production Chains (pp. 185-236). Academic Press. https://doi.org/10.1016/B978-0-12-814278-3.00007-8

• Booth, J., Miller, R., McKee, B., & Leathers, R. (2000). Wind-induced bottom sediment resuspension in a microtidal coastal environment. Continental Shelf Research, 20(7), 785-806. https://doi.org/10.1016/S0278-4343(00)00002-9

• Chen, Y., & Seybold, S. J. (2014). Crepuscular flight activity of an invasive insect governed by interacting abiotic factors. PLoS ONE, 9(8), Article e105945. https://doi.org/10.1371/journal.pone.0105945.

• Cheng, S., Munian, K., Sek-Aun, T., Faidi, M. A., & Ishak, S. F. (2020). Mitochondrial DNA diversity and gene flow in Southeast Asian populations of the synchronously flashing firefly, Pteroptyx tener Olivier (Coleoptera: Lampyridae). Oriental Insects, 54(2), 175-196. https://doi.org/10.1080/00305316.2019.1600594.

• Corbett, C. A. (2007). Colored Dissolved Organic Matter (CDOM) Workshop summary. Reports. 2. https://digitalcommons.usf.edu/basgp_report/2

• Du, C., Chen, J., Jiang, L., & Qiao, G. (2020). High correlation of species diversity patterns between specialist herbivorous insects and their specific hosts. Journal of Biogeography, 47(6), 1232-1245. https://doi.org/10.1111/jbi.13816

• Edde, P. A. (2022). Field crop arthropod pests of economic importance. Academic Press. https://doi.org/10.1016/B978-0-12-818621-3.00002-1

• Faudzi, R., Abas, A., Othman, N. W., & Mazlan, S. M. (2021). Effect of water quality on the abundance of firefly populations at Cherating River, Pahang, Malaysia. EnvironmentAsia, 14(1), 69-79. https://doi.org/10.14456/ea.2021.8

• Faust, L. F. (2004). Fireflies as a catalyst for science education. Integrative and Comparative Biology, 44(3), 264-265. https://doi.org/10.1093/icb/44.3.264

• Fazal, S., Manzoor, F., & Abdul-Sattar, A. (2012). Impact of abiotic factors on insect diversity of at Lawrence Garden, Lahore. Pakistan Journal of Science, 64(2), 127-132.

• Feng, Z., Su, B., Xiao, D. D., & Ye, L. Y. (2017). Study on pH value and its variation characteristics of the main rivers into Dianchi lake under the anthropogenic and natural processes, Yunnan, China. Journal of Information and Optimization Sciences, 38(7), 1197-1210. https://doi.org/10.1080/02522667.2017.1367501

• Fondriest Environmental. (2014). Conductivity, salinity and total dissolved solids. https://www.fondriest.com/environmental-measurements/parameters/ waterquality/conductivity-salinity-tds/

• Fu, X., Nobuyoshi, O., Vencl, F. V., & Lei, C. (2005). Structure, behavior, and the life cycle of an aquatic firefly, Luciola substriata, in China. The Canadian Entomologist, 137, 83-90. https://doi.org/10.4039/n04-022.

• Fu, X., Nobuyoshi, O., Vencl, F. V., & Lei, C. (2006). Life cycle and behaviour of the aquatic firefly Luciola leii (Coleoptera: Lampyridae) from Mainland China. Canadian Entomologist, 138(6), 860-870. https://doi.org/10.4039/n05-093

• Gatehouse, A. G. (1997). Behavior and ecological genetics of wind-borne migration by insects. Annual Review of Entomology, 42(1), 475-502. https://doi.org/10.1146/annurev.ento.42.1.475

• Gillett, D., & Marchiori, A. (2019). A low-cost continuous turbidity monitor. Sensors, 19(14), 3039. https://doi.org/10.3390/s19143039

• Hallmann, C. A., Sorg, M., Jongejans, E., Siepel, H., Hofland, N., Schwan, H., Stenmans, W., Müller, A., Sumser, H., Hörren, T., Goulson, D., & de Kroon, H. (2017). More than 75 percent decline over 27 years in total flying insect biomass in protected areas. PloS one, 12(10), Article e0185809. https://doi.org/10.1371/journal.pone.0185809

• Hazmi, I. R., & Sagaff, S. A. S. (2017). Fireflies population and the aquaculture industry (Coleoptera: Lampyridae) of the Sungai Sepetang, Kampung Dew, Perak, Malaysia. Serangga, 22(2), 217-237.

• Ho, J. Z., Chiang, P. H., Wu, C. H., & Yang, P. S. (2010). Life cycle of the aquatic firefly Luciola ficta (Coleoptera: Lampyridae). Journal of Asia-Pacific Entomology, 13(3), 189-196. https://doi.org/10.1016/j.aspen.2010.03.007

• Idris, N. S., Mustapha, M. A., Sulaiman, N., Khamis, S., Husin, S. M., & Darbis, N. D. A. (2021). The dynamics of landscape changes surrounding a firefly ecotourism area. Global Ecology and Conservation, 29, Article e01741. https://doi.org/10.1016/j.gecco.2021.e01741

• Jaikla, S., Lewis, S. M., Thancharoen, A., & Pinkaew, N. (2020). Distribution, abundance, and habitat characteristics of the congregating firefly, Pteroptyx Olivier (Coleoptera: Lampyridae) in Thailand. Journal of Asia-Pacific Biodiversity, 13(3), 358-366. https://doi.org/10.1016/j.japb.2020.05.002

• Jusoh, W. F. A. W., Hashim, N. R., & Ibrahim, Z. Z. (2010a). Firefly distribution and abundance on mangrove vegetation assemblages in Sepetang estuary, Peninsular Malaysia. Wetlands Ecology and Management, 18(3), 367-373. https://doi.org/10.1007/s11273-009-9172-4.

• Jusoh, W. F. A. W., Hashim, N. R., & Ibrahim, Z. Z. (2010b). Distribution and abundance of Pteroptyx fireflies in Rembau-Linggi estuary, Peninsular Malaysia. Environment Asia, 3(special issue), 56-60.

• Jusoh, W. F. A., Hashim, N. R., & Wong, C H. (2011). Zonation of firefly species and their display trees along Kerteh River, Terengganu. Serangga, 16(2), 59–66.

• Kaiser, L., Ode, P., van Nouhuys, S., Calatayud, P. A., Colazza, S., Cortesero, A. M., Thiel, A., & van Baaren, J. (2017). The plant as a habitat for entomophagous insects. Advances in Botanical Research, 18, 179–223. https://doi.org/10.1016/bs.abr.2016.09.006

• Keinath, S., Onandia, G., Griesbaum, F., & Rödel, M. O. (2023). Effects of urbanization, biotic and abiotic factors on aquatic insect diversity in urban ponds. Frontiers in Ecology and Evolution, 11, Article 1121400. https://doi.org/10.3389/fevo.2023.1121400

• Khoo, V., Nada, B., & Kirton, L. (2014, July 14-18). Conservation of the Selangor River population of Pteroptyx tener in Malaysia: Results of seven years of monitoring. In International Firefly Symposium 2014. Florida, USA.

• Khoo, V., Nada, B., Kirton, L. G., & Phon, C. K. (2012). Monitoring the population of the firefly Pteroptyx tener along the Selangor River, Malaysia for conservation and sustainable ecotourism. Lampyrid, 2, 162-173.

• Kirton, L. G., Nada, B., Tan, S. A., Ang, L. H., Tang, L. K., Hui, T. F., & Ho, W. M. (2006). The Kampung Kuantan firefly project: A preliminary assessment of the habitat requirements of Pteroptyx tener (Coleoptera: Lampyridae). Highlights of FRIM’s non IRPA projects. Forest Research Institute Malaysia, Malaysia.

• Koken, M., Guzmán-Álvarez, J. R., Gil-Tapetado, D., Romo Bedate, M. A., Laurent, G., Rubio, L. E., Comas, S. R, Wolffler, N., Verfaillie, F., & De Cock, R. (2022). Quick spreading of populations of an exotic firefly throughout Spain and their recent arrival in the French Pyrenees. Insects, 13(2), Article 148.

• Kokila, R., Balasubramanian, B., Meenambigai, K., Alagirisamy, K., Kumar, A., Kamyab, H., Ydav, K. K., & Nareshkumar, A. (2021). A GIS-based tool for the analysis of the distribution and abundance of Chilo sacchariphagus indicus under the influence of biotic and abiotic factors. Environmental Technology & Innovation, 21, Article 101357. https://doi.org/10.1016/j.eti.2021.101357

• Lewis, S. M., Wong, C. H., Owens, A., Fallon, C., Jepsen, S., Thancharoen, A., Wu. C., De Cock, R., Novák, M., López-Palafox, T., Khoo. V., & Reed, J. M. (2020). A global perspective on firefly extinction threats. BioScience, 70(2), 157-167. https://doi.org/10.1093/biosci/biz157

• Lloyd, J. E. (2008). Fireflies (Coleoptera: Lampyridae). In J. L. Capinera (Ed.), Encyclopedia of Entomology (pp. 1429–1452). Springer. https://doi.org/10.1007/0-306-48380-7

• Lubis, F. I., Agustin, I., Putra, G. I. S., Mailisa, I., Taufiq, Rahmah, S., Damayani, C. P. & Rudiansyah (2023). Male inflorescences, natural enemies, and climate as biotic and abiotic factors on population Elaeidobius kamerunicus at three soil types. In IOP Conference Series: Earth and Environmental Science (Vol. 1208, No. 1, p. 012007). IOP Publishing. https://doi.org/10.1088/1755-1315/1208/1/012007

• Mahadimenakbar, M. D., Jeperi, S. R., Fiffy Hanisdah, S., & Bagul, A. H. B. P. (2018). Notes on congregating fireflies (Coleoptera, Lampyridae) of Binsulok River, Sabah. Journal of Tropical Biology and Conservation, 15, 155-162.

• Malavasi, M. (2020). The map of biodiversity mapping. Biological Conservation, 252, Article 108843. https://doi.org/10.1016/j.biocon.2020.108843.

• , J. V., & Liebherr, J. K. (2009). Coleoptera:(beetles, weevils, fireflies). In Encyclopedia of insects (pp. 183-201). Academic Press. https://doi.org/10.1016/B978-0-12-374144-8.00062-X.

• Mileti, F. A., Russo, D., Fraissinet, M., Langella, G., Ferraro, G., & Terribile, F. (2024). Supporting the planning and management of biodiversity through the development of a geospatial decision support system. Land Degradation & Development, 35(5), 1837-1849. https://doi.org/10.1002/ldr.5024

• Moran, S. (2018). An applied guide to water and effluent treatment plant design. Elsevier. https://doi.org/10.1016/B978-0-12-811309-7.00023-0

• Nada, B., & Ballantyne, L. A. (2018). A new species of Pygoluciola Wittmer with unusual abdominal configuration, from lowland dipterocarp forest in peninsular Malaysia (Coleoptera Lampyridae: Luciolinae). Zootaxa, 4455(2), 343-362. https://doi.org/10.11646/zootaxa.4455.2.5

• Norela, S., Loo, M. C., & Abdullah, M. (2017). Association between the firefly population and some biotic and abiotic factors along the Sungai Sepetang river banks at Kampung Dew. Malayan Nature Journal, 69(3), 110-118.

• Obeidavi, Z., Rangzan, K., Kabolizade, M., & Mirzaei, R. (2019). A web-based GIS system for wildlife species: A case study from Khouzestan Province, Iran. Environmental Science and Pollution Research, 26(16), 16026-16039. https://doi.org/10.1007/s11356-019-04616-1

• Obilor, E. I., & Amadi, E. C. (2018). Test for significance of Pearson’s correlation coefficient. International Journal of Innovative Mathematics, Statistics & Energy Policies, 6(1), 11-23.

• Ohba, N., & Wong, C. H. (2004). External morphology and ecological study of the firefly, Pteroptyx tener at Kampong Kuantan, Selangor, Malaysia. Science Report of Yokosuka City Museum, 51, 1-33.

• Ozsahin, E., Ozdes, M., Smith, A. C., & Yang, D. (2022). Remote sensing and GIS-based suitability mapping of termite habitat in the African Savanna: A case study of the Lowveld in Kruger national park. Land, 11(6), Article 803. https://doi.org/10.3390/land11060803

• Palmer, T. A., Montagna, P. A., & Kalke, R. D. (2008). Benthic indicators of the initial effect of opening a channel. Environmental Bioindicators, 3(3-4), 205-206. https://doi.org/10.1080/15555270802551776

• Paramasivam, C. R. (2019). Merits and demerits of GIS and geostatistical techniques. In V. Senapathi, P. M. Viswanathan & S. Y. Chung (Eds.), GIS and Geostatistical Techniques for Groundwater Science Book (pp. 17-21). Elsevier. https://doi.org/10.1016/B978-0-12-815413-7.00002-X

• Peng, J., Kumar, K., Gross, M., Kunetz, T., & Wen, Z. (2020). Removal of total dissolved solids from wastewater using a revolving algal biofilm reactor. Water Environment Research, 92(5), 766-778. https://doi.org/10.1002/wer.1273

• Poukin, E., Mahadimenakbar, M. D., & Mohamed, M. (2023) The seasonal monsoon variations and the climatic effects on the abundance of fireflies (Coleoptera: Lampyridae) at Klias River, Beaufort, Sabah, East Malaysia. Serangga, 28(2),78-97.

• Prasertkul, T. (2018). Characteristics of Pteroptyx firefly congregations in a human dominated habitat. Journal of Insect Behavior, 31(4), 436-457. https://doi.org/10.1007/s10905-018-9687-8

• Rabha, M. M., Sharma, U., & Barua, A. G. (2021). Light from a firefly at temperatures considerably higher and lower than normal. Scientific Reports, 11(1), Article 12498. https://doi.org/10.1038/s41598-021-91839-3

• Reed, J. M., Nguyen, A., Owens, A. C., & Lewis, S. M. (2020). Linking the seven forms of rarity to extinction threats and risk factors: An assessment of North American fireflies. Biodiversity and Conservation, 29, 57-75. https://doi.org/10.1007/s10531-019-01869-7

• Riley, W. B., Rosa, S. P., & da Silveira, L. F. L. (2021). A comprehensive review and call for studies on firefly larvae. PeerJ, 9, Article e12121. https://doi.org/10.7717/peerj.12121

• Robertson-Bryan, I. (2004). pH requirements of freshwater aquatic life (pp. 1-13). Technical Memorandum, Robertson Bryan, Inc.

• Rocchini, D., Boyd, D. S., Féret, J. B., Foody, G. M., He, K. S., Lausch, A., Nagendra, H., Wegmann, M., & Pettorelli, N. (2016). Satellite remote sensing to monitor species diversity: Potential and pitfalls. Remote Sensing in Ecology and Conservation, 2(1), 25-36. https://doi.org/10.1002/rse2.9

• Roy, S., Barooah, A. K., Ahmed, K. Z., Baruah, R. D., Prasad, A. K., & Mukhopadhyay, A. (2020). Impact of climate change on tea pest status in northeast India and effective plans for mitigation. Acta Ecologica Sinica, 40(6), 432-442. https://doi.org/10.1016/j.chnaes.2019.08.003

• Saalidong, B. M., Aram, S. A., Otu, S., & Lartey, P. O. (2022). Examining the dynamics of the relationship between water pH and other water quality parameters in ground and surface water systems. PlosOne, 17(1), Article e0262117. https://doi.org/10.1371/journal.pone.0262117

• Sartsanga, C., Swatdipong, A., & Sriboonlert, A. (2018). Distribution of the fireflygenus Pteroptyx Olivier and a new record of Pteroptyx asymmetria Ballantyne (Coleoptera: Lampyridae: Luciolinae) in Thailand. The Coleopterists Bulletin, 72(1), 171-183. https://doi.org/10.1649/0010-065X-72.1.171

• Shahara, A., Nura, A. M. R., Maimon, A., & Norela, S. (2017). Assessment of firefly abundance at a new ecotourism site of Sungai Bernam, Selangor, Malaysia. Malayan Nature Journal, 69(2), 67-74.

• Smith, D., & Davis-Colley, R. (2001). Turbidity suspended sediment and water clarity. Journal of the American Water Resources Association, 37, 1085-1101. https://doi.org/10.1111/j.1752-1688.2001.tb03624.x

• Stevenson, M., & Bravo, C. (2019). Advanced turbidity prediction for operationalwater supply planning. Decision Support Systems, 119, 72-84. https://doi.org/10.1016/j.dss.2019.02.009

• Suzuki, J., Imamura, M., Nakano, D., Yamamoto, R., & Fujita, M. (2018). Effects of water turbidity and different temperatures on oxidative stress in caddisfly (Stenopsyche marmorata) larvae. Science of the Total Environment, 630, 1078-1085. https://doi.org/10.1016/j.scitotenv.2018.02.286

• Thancharoen, A. (2012). Well managed firefly tourism: A good tool for firefly conservation in Thailand. Lampyrid, 2, 142-148.

• Tokan, M. K., Imakulata, M. M., Neolaka, Y. A., & Kusuma, H. S. (2018). Species diversity and vertical distribution of arboreal organisms on the Paradiso mangrove environment of Kupang Bay, East Nusa Tenggara, Indonesia. Asian Journal of Agriculture and Biology, 6(4), 535-542.

• Tu, T. T., & Hoang, H. Y. (2023). Applications of Geographic Information System (GIS) in environmental management. Scientific Collection, (154), 397-402.

• Turney, S. (2022). Pearson correlation coefficient (r): Guide & examples. Scribbr. correlation-coefficient/.

• Usener, J. L., & Cognato, A. I. (2005). Patterns of mitochondrial diversity among desert firefly populations (Lampyridae: Microphotus octarthrus Fall). The Coleopterists Bulletin, 59(3), 361-367. https://doi.org/10.1649/796.1

• Van Swaay, C., van Strien, A., Harpke, A., Fontaine, B., Stefanescu, C., Roy, D., Kühn, E., Õnuao, E., Regan, E., Švitra, G., Prokofev, I., Heliölä, J., Settele, J., Pettersson, L., Botham, M., Musche, M., Titeux, N., Cornish, N., Leopold, P., … & Warren, M. (2013). The European grassland butterfly indicator: 1990–2011. EEA Technical Reports. https://doi.org/10.2800/89760

• Vaz, S., Guerrazzi, M. C., Rocha, M., Faust, L., Khattar, G., Mermudes, J., & da Silveira, L. F. L. (2021). On the intertidal firefly genus Micronaspis Green, 1948, with a new species and a phylogeny of Cratomorphini based on adult and larval traits (Coleoptera: Lampyridae). Zoologischer Anzeiger, 292, 64-91. https://doi.org/10.1016/j.jcz.2021.01.00

• Vaz, S., Manes, S., Gama‐Maia, D., Silveira, L., Mattos, G., Paiva, P. C., Figueirido, M., & Lorini, M. L. (2021). Light pollution is the fastest growing potential threat to firefly conservation in the Atlantic Forest hotspot. Insect Conservation and Diversity, 14(2), 211-224. https://doi.org/10.1111/icad.12481

• Wang, Y., Fu, X., Lei, C., Jeng, M. L., & Nobuyoshi, O. (2007). Biological characteristics of the terrestrial firefly Pyrocoelia pectoralis (Cleoptera: Lampyridae). The Coleopterists Bulletin, 61(1), 85-93. https://doi.org/10.1649/907.1

• Wong, C. H. (2022). Congregating Firefly Zones (CFZ) in Malaysia: Conservation of mangrove congregating fireflies. (MNS Conservation Publication No. 20). Malaysian Nature Society.

• Wong, C. H., & Yeap, C. A. (2012). Conservation of congregating firefly zones (CFZs) in Peninsular Malaysia. Lampyrid, 2, 174-187.

• Yahya, N., Idris, I., Rosli, N. S., & Bachok, Z. (2020). Mangrove-associated bivalves in Southeast Asia: A review. Regional Studies in Marine Science, 38, Article 101382. https://doi.org/10.1016/j.rsma.2020.101382

• Zhou, W. (2021). GIS for earth sciences. In D. Alderton & S. A. Elias (Eds.), Encyclopedia of Geology (pp. 281-293). Academic Press. https://doi.org/10.1016/B978-0-08-102908-4.00018-7