PERTANIKA JOURNAL OF SCIENCE AND TECHNOLOGY

 

e-ISSN 2231-8526
ISSN 0128-7680

Home / Regular Issue / JST Vol. 31 (4) Jul. 2023 / JST-3701-2022

 

Genetic Diversity of Asian Seabass (Lates calcarifer) in Captive Populations

Athirah Mohd Bakri and Yuzine Esa

Pertanika Journal of Science & Technology, Volume 31, Issue 4, July 2023

DOI: https://doi.org/10.47836/pjst.31.4.18

Keywords: captive, CO1 mtDNA, genetic distance, L. calcarifer

Published on: 3 July 2023

This study examined the genetic diversity of Asian seabass (Lates calcarifer) captive populations using sequencing of the mitochondrial DNA cytochrome c oxidase I (COI) fragment. The phylogenetic analyses of the 609 base pair regions of the COI fragment from 146 samples identified 15 haplotypes and divided them into two clades with a genetic divergence of 10%. Thus, phylogenetic results supported two genetic groups (the Australia/Southeast Asia group and the India/Myanmar group) within the captive populations under study. Mixed levels of genetic diversity were observed among captive populations, which indicated a certain degree of inbreeding depression. The findings would be useful for future aquaculture management of captive Asian seabass in Malaysia.

  • Das, S. P., Swain, S., Jena, J., & Das, P. (2018). Genetic diversity and population structure of Cirrhinus mrigala revealed by mitochondrial ATPase 6 gene. Mitochondrial DNA Part A: DNA Mapping, Sequencing, and Analysis, 29(4), 495-500. https://doi.org/10.1080/24701394.2017.1310852

  • Falush, D., Stephens, M., & Pritchard, J. K. (2003). Inference of population structure using multilocus genotype data: Linked loci and correlated allele frequencies. Genetics, 164(4), 1567-1587. https://doi.org/10.1093/genetics/164.4.1567

  • Froese, R., & Pauly, D. (Eds.). (2022). FishBase. World Wide Web electronic publication. http://www.fishbase.org

  • Grant, W. S., & Bowen, B. W. (1998). Obituary: Professor Michael Laskowski Sr. Acta Biochimica Polonica, 30(2), 113-114.

  • Hebert, P. D. N., Cywinska, A., Ball, S. L., & DeWaard, J. R. (2003). Biological identifications through DNA barcodes. Proceedings of the Royal Society B: Biological Sciences, 270(1512), 313-321. https://doi.org/10.1098/rspb.2002.2218

  • Idris, S. M., Noordin, W. N. M., Manah, F. O., & Hamzah, A. (2022). Toward systematic breeding of asian sea bass, Lates calcarifer (Bloch, 1790), in Malaysia: status, challenges and prospects for future development. Asian Fisheries Science, 35(1), 1-12. https://doi.org/10.33997/j.afs.2022.35.1.001

  • Kumar, S., Stecher, G., & Tamura, K. (2016). MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33(7), 1870-1874. https://doi.org/10.1093/molbev/msw054

  • Norfatimah, M. Y., Azizah, M. N. S., Othman, A. S., Patimah, I., & Jamsari, A. F. J. (2009). Genetic variation of Lates calcarifer in Peninsular Malaysia based on the cytochrome b gene. Aquaculture Research, 40(15), 1742-1749. https://doi.org/10.1111/j.1365-2109.2009.02279.x

  • Senanan, W., Pechsiri, J., Sonkaew, S., Na-Nakorn, U., Sean-In, N., & Yashiro, R. (2014). Genetic relatedness and differentiation of hatchery populations of Asian seabass (Lates calcarifer) (Bloch, 1790) broodstock in Thailand inferred from microsatellite genetic markers. Aquaculture Research, 46(12), 2897-2912. https://doi.org/10.1111/are.12442

  • Vij, S., Purushothaman, K., Gopikrishna, G., Lau, D., Saju, J. M., Shamsudheen, K. V., Vinaya Kumar, K., Basheer, V. S., Gopalakrishnan, A., Hossain, M. S., Sivasubbu, S., Scaria, V., Jena, J. K., Ponniah, A. G., & Orbán, L. (2014). Barcoding of Asian seabass across its geographic range provides evidence for its bifurcation into two distinct species. Frontiers in Marine Science, 1, Article 30. https://doi.org/10.3389/fmars.2014.00030

  • Ward, R. D., Holmes, B. H., & Yearsley, G. K. (2008). DNA barcoding reveals a likely second species of Asian sea bass (barramundi) (Lates calcarifer). Journal of Fish Biology, 72(2), 458-463. https://doi.org/10.1111/j.1095-8649.2007.01703.x

  • Ward, R. D, Zemlak, T. S., Innes, B. H., Last, P. R., & Hebert, P. D. N. (2005). DNA barcoding Australia’s fish species. Philosophical Transactions of the Royal Society B: Biological Sciences, 360(1462), 1847-1857. https://doi.org/10.1098/rstb.2005.1716

  • Zhang, Q., Sun, C., Zhu, Y., Xu, N., & Liu, H. (2020). Genetic diversity and structure of the round-tailed paradise fish (Macropodus ocellatus): Implications for population management. Global Ecology and Conservation, 21(159), Article e00876. https://doi.org/10.1016/j.gecco.2019.e00876

  • Zhu, Z. Y., Lin, G., Lo, L. C., Xu, Y. X., Feng, F., Chou, R., & Yue, G. H. (2006). Genetic analyses of Asian seabass stocks using novel polymorphic microsatellites. Aquaculture, 256(1-4), 167-173. https://doi.org/10.1016/j.aquaculture.2006.02.033

ISSN 0128-7680

e-ISSN 2231-8526

Article ID

JST-3701-2022

Download Full Article PDF

Share this article

Related Articles